PoplarV1, Main, Exploration, bibRecord, 004471

Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides.

Identifieur interne : 004471 ( Main/Exploration ); précédent : 004470; suivant : 004472

Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides.

Auteurs : Patrik Johansson [Suède] ; Stuart Denman ; Harry Brumer ; Asa M. Kallas ; Hongbin Henriksson ; Terese Bergfors ; Tuula T. Teeri ; T Alwyn Jones

Source :

Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 2003.

RBID : pubmed:12595718

Descripteurs français

KwdFr :
- Arbres (enzymologie), Clonage moléculaire (MeSH), Conformation des protéines (MeSH), Cristallisation (MeSH), Cristallographie aux rayons X (MeSH), Glycosyltransferase (composition chimique), Glycosyltransferase (isolement et purification), Indicateurs et réactifs (MeSH), Protéines recombinantes (composition chimique).
MESH :
- composition chimique : Glycosyltransferase, Protéines recombinantes.
- enzymologie : Arbres.
- isolement et purification : Glycosyltransferase.
- Clonage moléculaire, Conformation des protéines, Cristallisation, Cristallographie aux rayons X, Indicateurs et réactifs.

English descriptors

KwdEn :
- Cloning, Molecular (MeSH), Crystallization (MeSH), Crystallography, X-Ray (MeSH), Glycosyltransferases (chemistry), Glycosyltransferases (isolation & purification), Indicators and Reagents (MeSH), Protein Conformation (MeSH), Recombinant Proteins (chemistry), Trees (enzymology).
MESH :
- chemical , chemistry : Glycosyltransferases, Recombinant Proteins.
- chemical , isolation & purification : Glycosyltransferases.
- enzymology : Trees.
- Cloning, Molecular, Crystallization, Crystallography, X-Ray, Indicators and Reagents, Protein Conformation.

Abstract

Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 A resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 A) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 A).

DOI: 10.1107/s090744490202348x
PubMed: 12595718

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>Glycosyltransferases (isolation & purification)</term>
<term>Indicators and Reagents (MeSH)</term>
<term>Protein Conformation (MeSH)</term>
<term>Recombinant Proteins (chemistry)</term>
<term>Trees (enzymology)</term>
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<term>Clonage moléculaire (MeSH)</term>
<term>Conformation des protéines (MeSH)</term>
<term>Cristallisation (MeSH)</term>
<term>Cristallographie aux rayons X (MeSH)</term>
<term>Glycosyltransferase (composition chimique)</term>
<term>Glycosyltransferase (isolement et purification)</term>
<term>Indicateurs et réactifs (MeSH)</term>
<term>Protéines recombinantes (composition chimique)</term>
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<term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Indicators and Reagents</term>
<term>Protein Conformation</term>
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<term>Conformation des protéines</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Indicateurs et réactifs</term>
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<front><div type="abstract" xml:lang="en">Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 A resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 A) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 A).</div>
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<Month>10</Month>
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<Abstract><AbstractText>Xyloglucan endotransglycosylases (XETs) cleave and religate xyloglucan polymers in plant cell walls. Recombinant XET from poplar has been purified from a Pichia pastoris expression system and crystallized. Two different crystal forms were obtained by vapour diffusion from potassium sodium tartrate and from an imidazole buffer using sodium acetate as a precipitant. Data were collected from these crystal forms to 3.5 and 2.1 A resolution, respectively. The first crystal form was found to belong to space group P3(1)21 or P3(2)21 (unit-cell parameters a = 98.6, b = 98.6, c = 98.5 A) and the second crystal form to space group P6(3) (unit-cell parameters a = 188.7, b = 188.7, c = 46.1 A).</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Johansson</LastName>
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<country name="Suède"><region name="Svealand"><name sortKey="Johansson, Patrik" sort="Johansson, Patrik" uniqKey="Johansson P" first="Patrik" last="Johansson">Patrik Johansson</name>
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Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides.

Crystallization and preliminary X-ray analysis of a xyloglucan endotransglycosylase from Populus tremula x tremuloides.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki